Localization of senescence marker protein-30 (SMP30) in the mouse submandibular gland and histological findings in the SMP30 knock-out mouse

摘要

Senescence marker protein-30 (SMP30), which has a molecular mass of 30 kDa, is strongly expressed during maturation and decreases with aging in the liver and kidney. SMP30 is suspected of being closely related to cell functions, especially intracellular Ca2+ homeostasis, in differentiated tissues of the liver and kidney. We produced an antibody against SMP30 to study the localization of SMP30 in the submandibular gland of normal mice using an enzyme-labeled antibody. The submandibular gland was also observed under transmission electron microscopy in newly created SMP30-knockout mice compared to normal mice. SMP30 was found to be widely distributed in acinic cells of ductal and peripheral areas of the submandibular gland. Degenerative mitochondria tended to be abundantly observed in cells with SMP30, although marked changes were not noted in SMP30-knockout mice.