Development of 16S rRNA targeted PCR for the identification of Vibrio spp., the causative bacteria of the disease in cultured sea urchin Strongylocentrotus intermedius occurring at low water temperatures [Japanese]

摘要

The causative bacteria, Vibrio spp., have been isolated from the diseased sea urchin Strongylocentrotus intermedius at low water temperatures. To develop a specific identification method for the pathogens, species-specific nucleotide sequences in the 16S rRNA of Vibrio spp., were determined. We designed two PCR primers, DA2F and SK1F, based on the nucleotide sequences: DA2F forward primer (nucleotide numbers 456 to 476 in Escherichia coli 16S rRNA) for Date isolates and Sk1F for-ward primer (nucleotide numbers 455 to 478 in E. coli 16S rRNA) for Shiriuchi and Shikabe isolates.