Introduction of human leukocytes in cold anabiosis (-20 degrees C) under an exponential program

摘要

Investigation of effective and accessible method for administration of human blood leukocytes in the cold anabiosis condition at am oderately low temperature of minus 20 degrees C by the exponential program with cryopreservative which does not require washing from the bio-object after its thawing, has been proposed. Morphological and functional properties of the leukocytes after different periods of conservation in the state of cold anabiosis were studied. The data obtained suggest that optimal conservation of the morpho-functional properties of cells with the aid of this method equals 3 weeks.