In Vitro Effects of Human Tooth Bone Graft Materials on RAW264.7 Cell Biological Behavior

摘要

Objective: To determine the bio-compatibility and inflammatory response of human tooth bone graft material prepared from autologous human teeth on the murine macrophage cell line RAW264.7. Methods: Morphological characteristics of the human tooth bone graft material, OSTEON II and untreated tooth granules were observed by scanning electron microscopy. RAW264.7 cells were co-cultured with the different materials, and changes in viability and morphology were observed under a confocal microscope. The cells were also cultured with the extracts of the different graft materials, and the growth curve, apoptosis, and IL-6 and TNF-alpha production and secretion were measured by Trypan blue staining, Annexin V staining, Western blotting and enzyme-linked immunosorbent assay (ELISA) respectively. Results: The human tooth bone graft materials showed favorable scaffold structure, and did not affect the morphology, proliferation, differentiation and apoptosis of the macrophages. The extract of the autologous graft material also did not increase the production and secretion of TNF-alpha and IL-6. Conclusion: Human tooth bone graft material was bio-compatible and showed low risk of inducing an inflammatory response, indicating their potential in regenerative dental surgery.