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首页> 外文期刊>Limnology and oceanography, methods >Running the Stop Sign: Readthrough of a Premature UAG Termination Signal in the Translation of a Zebrafish (Danio rerio) Taurine Biosynthetic Enzyme
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Running the Stop Sign: Readthrough of a Premature UAG Termination Signal in the Translation of a Zebrafish (Danio rerio) Taurine Biosynthetic Enzyme

机译:运行停止标志:在斑马鱼(Danio Rerio)Taurine Biosynthetic酶翻译中的过早UAG终端信号的推感

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摘要

The UAG termination codon is generally recognized as the least efficient and least frequently used of the three universal stop codons. This is substantiated by numerous studies in an array of organisms. We present here evidence of a translational readthrough of a mutant nonsense UAG codon in the transcript from the cysteine sulfinic acid decarboxylase (csad) gene (ENSDARG00000026348) in zebrafish. The csad gene encodes the terminal enzyme in the taurine biosynthetic pathway. Taurine is a critical amino acid for all animals, playing several essential roles throughout the body, including modulation of the immune system. The sa9430 zebrafish strain (ZDB-ALT-130411-5055) has a point mutation leading to a premature stop codon (UAG) 20 amino acids 5' of the normal stop codon, UGA. Data from immunoblotting, enzyme activity assays, and mass spectrometry provide evidence that the mutant is making a CSAD protein identical to that of the wild-type (XP_009295318.1) in terms of size, activity, and amino acid sequence. UAG readthrough has been described in several species, but this is the first presentation of a case in fish. Also presented are the first data substantiating the ability of a fish CSAD to utilize cysteic acid, an alternative to the standard substrate cysteine sulfinic acid, to produce taurine.
机译:UAG终端密码子通常被识别为三个通用阻止密码子的最低有效和最常用。这是一系列生物体中的许多研究证实。这里我们在这里呈现了斑马鱼中半胱氨酸亚硫酸脱羧酶(CSAD)基因(CSADARG000026348)的转录物中的突变型无义义氏芯榫的转化读取。 CSAD基因编码牛磺酸生物合成途径中的末端酶。牛磺酸是所有动物的关键氨基酸,在整个身体上发挥几种必需的作用,包括调节免疫系统。 SA9430斑马鱼菌株(ZDB-ALT-130411-5055)的点突变导致正常止墩封密码子的过早止芯(UAG)20氨基酸5'。免疫印迹的数据,酶活性测定和质谱提供了证据表明突变体在大小,活性和氨基酸序列方面使突变体能够与野生型(XP_009295318.1)相同的CSAD蛋白。 uag推高已经在几种物种中描述,但这是FISH中的案例的第一次呈现。还提出的是第一数据证实鱼CSAD利用浑囊酸的能力,是标准底物半胱氨酸硫酸的替代品,以产生牛磺酸。

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