首页> 外文期刊>Analytical methods >Combining cross-reactivity of an electrode array with the selective thiol reporting process of redox indicators: targeted sensing of biothiols
【24h】

Combining cross-reactivity of an electrode array with the selective thiol reporting process of redox indicators: targeted sensing of biothiols

机译:将电极阵列的交叉反应与氧化还原指标的选择性硫醇报告过程结合起来:生物醇的靶向感测

获取原文
获取原文并翻译 | 示例
           

摘要

This paper describes the syntheses, characterization, and electro-analytical applications of metallophthalocyanine and metallophthalocyanine embedded GO/rGO materials, named CuPc (1), MnPc (2), GO-CuPc-PDA (3), GO-MnPc-PDA (4), rGO-CuPc-PDA (5), and rGO-MnPc-PDA (6) (where CuPc = copper phthalocyanine, MnPc = manganese phthalocyanine, GO = graphene oxide, rGO = reduced graphene oxide, and PDA = polydopamine). The synthesized materials were dip coated over a glassy carbon electrode (GCE) surface to yield an array of six electrodes (1'-6'). Each of the electrodes has shown distinct voltammetric oxidation signals towards different biothiols, such as cysteine (Cys), glutathione (GSH), and homocysteine (Hcys). The collective response of the electrode array was analyzed using chemometric analysis, such as linear discriminant analysis (LDA), which enables the simultaneous detection of Cys, GSH, and Hcys in a phosphate buffer. However, the application of the electrode array technique to the biomarker detection is often complicated due to the non-specific interference of protein components present in biofluids. We demonstrate a proof-of-principle that such interference in discriminatory sensing of thiols can be circumvented via combining the cross-reactivity of the electrode array with the selective thiol reporting process of redox indicators (RIs), such as ascorbic acid (AA), dopamine (DA), and uric acid (UA).
机译:本文介绍了金属酞菁和金属酞菁嵌入式GO / RGO材料,名为Cupc(1),MNPC(2),Go-MNPC-PDA(4 ),Rgo-ChC-PDA(5)和RGO-MNPC-PDA(6)(其中Cupc =铜酞菁,MNPC =锰酞菁,GO =石墨烯氧化物,RGO =还原氧化物,和PDA =多多胺)。将合成的材料浸涂在玻璃状碳电极(GCE)表面上,以产生六个电极(1'-6')的阵列。每个电极已经显示出不同的伏安氧化信号,朝向不同的生物醇,例如半胱氨酸(Cys),谷胱甘肽(GSH)和同型半胱氨酸(HCYS)。使用化学计量分析分析电极阵列的集体响应,例如线性判别分析(LDA),其能够在磷酸盐缓冲液中同时检测Cys,GSH和Hcys。然而,由于生物流体中存在的蛋白质成分的非特异性干扰,电极阵列技术施加到生物标志物检测通常是复杂的。我们证明了一种原则上,可以通过将电极阵列的交叉反应与氧化还原指标(RIS)的选择性硫醇报告过程组合,例如抗坏血酸(AA)的选择性硫醇报告过程来避免硫醇的歧视性感测的原则上的原则上的这种干扰。多巴胺(DA)和尿酸(UA)。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号