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首页> 外文期刊>Chembiochem: A European journal of chemical biology >Site‐Selective Functionalization of Flagellin by Steric Self‐Protection: A Strategy To Facilitate Flagellin as a Self‐Adjuvanting Carrier in Conjugate Vaccine
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Site‐Selective Functionalization of Flagellin by Steric Self‐Protection: A Strategy To Facilitate Flagellin as a Self‐Adjuvanting Carrier in Conjugate Vaccine

机译：鞭毛素通过空间自我保护的场地选择性官能化：一种促进鞭毛蛋白作为共轭疫苗的自助载体的策略

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Abstract > Flagellin (FliC) can act as a carrier protein in the preparation of conjugate vaccines to elicit a T‐cell‐dependent immune response and as an intrinsic adjuvant to activate the toll‐like receptor?5 (TLR5) to enhance vaccine potency. To enable the use of FliC as a self‐adjuvanting carrier, an effective method for site‐selective modification (SSM) of pertinent amino‐acid residues in the D2 and D3 domains of FliC is explored without excessive modification of the D0 and D1 domains, which are responsible for activating and binding with TLR5. In highly concentrated Na <sub>2</sub> SO <sub>4</sub> solution, FliC monomers form flagellar filaments, in which the D0 and D1 domains are situated inside the tubular structure. Thus, the lysine residues (K219, K224, K324, and K331) in the D2 and D3 domains of flagellin are selectively modified by a diazo‐transfer reaction with imidazole‐1‐sulfonyl azide. The sites with azido modification are confirmed by MALDI‐TOF‐MS, ESI‐TOF‐MS, and LC–MS/MS analyses along with label‐free quantitation. The azido‐modified filament dissolves to give FliC monomers, which can conjugate with alkyne‐hinged saccharides by the click reaction. Transmission electron microscopy imaging, dynamic light scattering measurements, and the secreted embryonic alkaline phosphatase reporter assay indicate that the modified FliC monomers retain the ability either to bind with TLR5 or to reassemble into filaments. Overall, this study establishes a feasible method for the SSM of FliC by steric self‐protection of the D0 and D1 domains. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >鞭毛蛋白（Flic）可以作为载体蛋白在制备共轭疫苗中引出一个载体​​蛋白T细胞依赖性免疫应答和作为固有佐剂，以激活Toll样受体β5（TLR5）以增强疫苗效力。为了使得Flic作为自佐剂载体的使用，探讨了在D2和D3域中的D2和D3结构域中有关氨基酸残基的有效方法的有效方法，而不会过度修饰D0和D1结构域，这负责与TLR5激活和结合。在高浓度Na <sub> 2 </ sub> SO <sub> 4 </ sub>溶液中，叶片单体形成鞭毛长丝，其中D0和D1结构域位于管状结构内。因此，通过与咪唑-1-磺酰基叠氮化物的重氮转移反应选择性地改变鞭毛蛋白D2和D3结构域中的赖氨酸残基（K219，K224，K324和K331）。具有Azido修饰的位点由MALDI-TOF-MS，ESI-TOF-MS和LC-MS / MS分析以及无标签定量进行了分析。 Azido改性的长丝溶解过味道单体，其可以通过点击反应与炔肾血清糖缀合。透射电子显微镜成像，动态光散射测量和分泌的胚胎碱性磷酸酶报告结果表明改性的叶片单体保留与TLR5结合或将其重新组织成长丝的能力。总体而言，该研究通过D0和D1结构域的空间自我保护来确定Flic的SSM的可行方法。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16039/'>《Chembiochem: A European journal of chemical biology》</a> <b style="margin: 0 2px;">|</b><span>2018年第8期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Peng Chi‐Jiun&option=202" target="_blank" rel="nofollow">Peng Chi‐Jiun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chen Hsiu‐Ling&option=202" target="_blank" rel="nofollow">Chen Hsiu‐Ling;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chiu Cheng‐Hsun&option=202" target="_blank" rel="nofollow">Chiu Cheng‐Hsun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fang Jim‐Min&option=202" target="_blank" rel="nofollow">Fang Jim‐Min;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of ChemistryNational Taiwan University1 Sec. 4 Roosevelt Road Taipei 10617 Taiwan;</p> <p>Molecular Infectious Disease Research CenterChang Gung Memorial Hospital5 Fuxing Street Guishan District Taoyuan 33302 Taiwan;</p> <p>Molecular Infectious Disease Research CenterChang Gung Memorial Hospital5 Fuxing Street Guishan District Taoyuan 33302 Taiwan;</p> <p>Department of ChemistryNational Taiwan University1 Sec. 4 Roosevelt Road Taipei 10617 Taiwan;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=antigens&option=203" rel="nofollow">antigens;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=conjugate vaccines&option=203" rel="nofollow">conjugate vaccines;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=flagellins&option=203" rel="nofollow">flagellins;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=self-assembly&option=203" rel="nofollow">self-assembly;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=steric hindrance&option=203" rel="nofollow">steric hindrance;</a> </p> <div class="translation"> 机译：抗原;共轭疫苗;鞭毛;自我组装;空间障碍; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022056213.html">Front Cover: Site‐Selective Functionalization of Flagellin by Steric Self‐Protection: A Strategy To Facilitate Flagellin as a Self‐Adjuvanting Carrier in Conjugate Vaccine (ChemBioChem 8/2018)</a> <b>[J]</b> . <span> <a 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<b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704019346207.html">Salmonella flagellin is a potent carrier-adjuvant for peptide conjugate to induce peptide-specific antibody response in mice</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Qian Feng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Qian Feng,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guo Aihua&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Guo Aihua,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Mengmeng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Li Mengmeng,</a> <a href="/journal-foreign-32795/" target="_blank" rel="nofollow" class="tuijian_authcolor">Vaccine .</a> <span>2015</span><span>,第17期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: 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.</a> <span>2015</span><span>,第2期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：鞭毛蛋白作为可卡因疫苗开发的载体和佐剂</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_16th-international-congress-animal-hygiene_thesis/020511103583.html">Monomeric Flagellin May be More Suitable as an Adjuvant Than Polymeric Flagellin</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hu Maozhi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hu Maozhi,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pan Zhiming&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Pan Zhiming,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Yun&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yang Yun,</a> <a href="/conference-foreign-15505/" target="_blank" rel="nofollow" class="tuijian_authcolor">16th International congress on animal hygiene : animal hygiene, health and welfare as corner stones of sustainable animal production .</a> <span>2013</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：单体鞭毛蛋白可能比聚合鞭毛蛋白更适合用作佐剂</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061186790.html">Flagellin is an effective mucosal adjuvant for immunization against lethal respiratory challenge with Yersinia pestis.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Honko, Anna N.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Honko, Anna N. </a> <span>2005</span> </span> </div> <p class="zwjiyix 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Chang,</a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于鞭毛蛋白载体的蛋白纳米颗粒疫苗与流感保守表位融合可提供针对甲型流感病毒攻击的全面保护</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000126310724.html">Front Cover: Site-Selective Functionalization of Flagellin by Steric Self-Protection: A Strategy To Facilitate Flagellin as a Self-Adjuvanting Carrier in Conjugate Vaccine (ChemBioChem 8/2018)</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chi-Jiun Peng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Chi-Jiun Peng,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hsiu-Ling Chen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hsiu-Ling Chen,</a> <a 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