Protein Crystallography and Site‐Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from        <i >Ideonella sakaiensisIdeonella sakaiensis

Liu Bing; He Lihui; Wang Liping; Li Tao; Li Changcheng; Liu Huayi; Luo Yunzi; Bao Rui

摘要

Abstract > Unlike traditional recycling strategies, biodegradation is a sustainable solution for disposing of poly(ethylene terephthalate) (PET) waste. PETase, a newly identified enzyme from Ideonella sakaiensis , has high efficiency and specificity towards PET and is, thus, a prominent candidate for PET degradation. On the basis of biochemical analysis, we propose that a wide substrate‐binding pocket is critical for its excellent ability to hydrolyze crystallized PET. Structure‐guided site‐directed mutagenesis revealed an improvement in PETase catalytic efficiency, providing valuable insight into how the molecular engineering of PETase can optimize its application in biocatalysis. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>摘要</ title> >与传统的回收策略不同，生物降解是处理聚（乙烯对苯二甲酸乙二醇酯）的可持续解决方案（宠物）浪费。 Petase，来自 ideonella sakaiensis的新鉴定的酶</ i>对PET具有高效率和特异性，因此是宠物降解的突出候选者。在生物化学分析的基础上，我们提出宽的基板结合口袋对于其优异的水解结晶宠物的能力至关重要。结构引导的部位定向诱变揭示了帕萨斯催化效率的提高，提供了有价值的洞察，以如何优化其在生物催化中的应用。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16039/'>《Chembiochem: A European journal of chemical biology》</a> <b style="margin: 0 2px;">|</b><span>2018年第14期</span><b style="margin: 0 2px;">|</b><span>共5页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Bing&option=202" target="_blank" rel="nofollow">Liu Bing;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=He Lihui&option=202" target="_blank" rel="nofollow">He Lihui;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Liping&option=202" target="_blank" rel="nofollow">Wang Liping;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Tao&option=202" target="_blank" rel="nofollow">Li Tao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Changcheng&option=202" target="_blank" rel="nofollow">Li Changcheng;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Huayi&option=202" target="_blank" rel="nofollow">Liu Huayi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Luo Yunzi&option=202" target="_blank" rel="nofollow">Luo Yunzi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bao Rui&option=202" target="_blank" rel="nofollow">Bao Rui;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of GastroenterologySichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041 China;</p> <p>Center of Infectious DiseasesSichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041 China;</p> <p>Department of GastroenterologySichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041 China;</p> <p>Center of Infectious DiseasesSichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041 China;</p> <p>Center of Infectious DiseasesSichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041 China;</p> <p>Department of GastroenterologySichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041 China;</p> <p>Department of GastroenterologySichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041 China;</p> <p>Center of Infectious DiseasesSichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041 China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=mutagenesis&option=203" rel="nofollow">mutagenesis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=polymers&option=203" rel="nofollow">polymers;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=protein engineering&option=203" rel="nofollow">protein engineering;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=sustainable chemistry&option=203" rel="nofollow">sustainable chemistry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=waste disposal&option=203" rel="nofollow">waste disposal;</a> </p> <div class="translation"> 机译：诱变;聚合物;蛋白质工程;可持续化学;废物处理; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022055288.html">Cover Feature: Protein Crystallography and Site‐Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from <i >Ideonella sakaiensisIdeonella sakaiensis (ChemBioChem 14/2018)</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Bing&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Liu Bing,</a> <a 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Protein Crystallography and Site‐Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from Ideonella sakaiensisIdeonella sakaiensis

摘要