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Histological and immunohistochemical studies of the structure of lymph nodes in Kilis goats

机译:基尔斯山羊淋巴结结构的组织学和免疫组织化学研究

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Ten healthy adult Kilis goat mesenteric lymph nodes were used to examine the general structure of lymph nodes, lymphocytes, plasma cells, reticular cells and reticular fibers using histological methods. We also detected T lymphocytes using anti-CD3 [SP7], anti-CD4 [74-12-4], mouse anti-bovine CD4 [CC30] and mouse anti-bovine CD8 [CC63] monoclonal antibodies (mAb); and B lymphocytes using anti-CD79a [HM57] mAb, macrophages using anti-macrophage [MAC387] mAb and follicular dendritic cells using anti-S100 polyclonal antibody (pAb). The distribution of these cells also was studied. Although the primer antibodies we used for CD3, CD8, CD79a, MAC387 and S100 worked well, the primer antibodies for CD4 were ineffective for paraffin embedded goat lymph nodes.
机译:使用组织学方法使用10个健康成人的岩浆肠系膜肠系膜淋巴结,用于检查淋巴结,淋巴细胞,血浆细胞,网状细胞和网状纤维的一般结构。 我们还使用抗CD3 [SP7]检测到T淋巴细胞,抗CD4 [74-12-4],小鼠抗牛CD4 [CC30]和小鼠抗牛CD8 [CC63]单克隆抗体(MAB); 使用抗CD79A [HM57] MAB的B淋巴细胞,使用抗巨噬细胞[MAC387] MAB和卵泡树突细胞使用抗S100多克隆抗体(PAB)。 研究了这些细胞的分布。 虽然我们用于CD3,CD8,CD79a,Mac387和S100的引物抗体很好,但CD4的引物抗体对于石蜡嵌入山羊淋巴结无效。

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