In vitro organogenesis of Anoectochilus formosanaus under different sources of lights

摘要

Light has a profound effect on plant growth and development. Light-emitting diodes (LEDs) represent a fundamentally different technology from the gaseous discharge-type lamps currently used in horticulture, and LEDs have a number of advantages when compared to traditional forms of lighting. For horticultural researchers and crop producers to benefit from LED use, a variety of preliminary findings should be considered. Anoectochilus formosanaus {Orchidaceae) is an important medicinal herb that flowers only once in a year. The whole plant of A. formosanaus, fresh or dried, is boiled in water and taken orally as medicine. The objective of this study was to develop new procedures of rapid production of A. formosanaus from the shoot tip of the plants by using different sources of lights. Shoot tips of A. formosanaus were cultured with modified Murashige and Skoog medium and all cultures were maintained under five different sources of lights (white fluorescent tube, blue LED, red LED, green LED, and Red field emission lamp or Red FEL) with a 16-h photoperiod for 6 weeks Our experimental results indicate that green LED promotes the maximum number of branches, leaves and roots without the use of any plant growth regulator. Fresh weight (158.5 mg) and maximumformation rate of branches (92%) and roots (56%) were observed under green LED compared with the other four sources of light. Reports from this study suggest an easy, fast and reliable in vitro regeneration system for the organogenesis of A. formosanaus.