Identification of RAPD marker linked to resistance of Musa to Fusarium oxysporum

摘要

Banana and plantain (Musa spp.) are grown in more than 120 countries. They are cheap to produce, grow in a range of environments, and produce fruit year-round. Fusarium wilt of banana is widely regarded as one of the most destructive plant diseases. It is caused by soil-borne fungus Fusarium oxysporum f. sp. cubense. There is no chemical control available and the only way is to plant cultivars resistant/tolerant to the pathogen. In this study, we report a RAPD marker linked to susceptibility and resistance of Musa to F. oxysporum. Eight susceptible and ten resistant/tolerant accessions evaluated in field were used for this study. Genomic DNA was isolated from fully expanded young leaves. The RAPD patterns obtained with the primer OPK-03 detected a major fragment of 485 bp observed in the susceptible entrances, but absent in the resistant/tolerant entrances. The study of genetic relations between the genotypes susceptible and resistant/tolerant to F. oxysporum, using RAPD primers, defined the existence of an associated polymorphic pattern to the resistance..