CULTURAL REQUIREMENTS FOR MAXIMUM CONIDIAL PRODUCTION OF CERCOSPORA KIKUCHII, THE CAUSE OF PURPLE SEED STAIN OF SOYBEAN

摘要

The effect of different types of medium, pH, exposure to light and temperature on conidial production of Ccrcospora kikuchii was studied. Abundant in vitro sporulation of the fungus was recorded in mungbean seed decoction agar followed by soybean leafdecoction agar and V-8 juice agar after 14 days of incubation. Maximum sporulation was noted at pH 6.0 - 6.6, at 25 deg C, exposure to 12-hr light and 12-hr darkness daily and lowest sporulation in soybean seed decoction agar, pH 7.5 at 25 deg C andat 35 deg C at pH 6.5 under continuous light.