Ultrastructural study of the cheek oral mucosa of rats submitted to experimental chronic alcoholism.

摘要

Ultrastructural features of the cheek oral mucosa of rats (Rattus norvegicus) submitted to experimental chronic alcoholism were studied by transmission electron microscopy. Sixty male rats aged three months and with a mean body weight of 250 g were divided into two experimental groups. The control group received a solid diet (Purina rat chow) and tap water ad libitum. The alcoholic group received the same solid diet but was only allowed to drink sugar cane liquor dissolved in 30 degrees Gay Lussac (v/v). The animals of the two groups were weighed and sacrificed after 60, 120, and 180 days of treatment. Samples of the oral mucosa from the cheek region were dissected and processed for ultrastructural analysis. The results showed cell alterations in the basal and intermediate layer of the mucosal epithelium. The most frequent alterations observed were an increased intercellular space, the presence of lipid droplets in the cytoplasm, and irregular nuclei with a pyknotic aspect.