Kupffer cell activation by ambient air particulate matter exposure may exacerbate non-alcoholic fatty liver disease

摘要

Owing to increased obesity, non-alcoholic fatty liver disease (NAFLD) is now the most prevalent liver disease in the United States. NAFLD is considered a component of metabolic syndrome, a cluster of disorders that also includes diabetes mellitus, dyslipidemia, arteriosclerosis, and hypertension. Exposure to ambient air particulate matter with aerodynamic diameters2.5μm (PM2.5) is a risk factor for arteriosclerosis and lung disease, but its effect on NAFLD is unknown. PM2.5 induces pulmonary dysfunction via Toll-like receptor (TLR) activation on alveolar macrophages. TLR activation of Kupffer cells, resident hepatic macrophages, and subsequent pro-inflammatory cytokine production have been shown to play a key role in NAFLD progression. We hypothesized that PM2.5 exposure is a significant risk factor for the progression of NAFLD. Thus, following exposure of male C57BL/6 mice fed high fat chow (HFC) to concentrated air particulate matter (CAPs) or filtered air for 6 weeks, progression of NAFLD was evaluated by standardized histological assessment of hepatic inflammation and fibrosis. In mice fed HFC, the hepatic inflammatory grade (3.00±0.00 vs. 1.50±0.71, P0.001) and fibrosis stage (1.00±0.00 vs. 0.60±0.52, P0.023) were both significantly higher in mice exposed to CAPs versus filtered air, respectively. Increased numbers of Kupffer cells contained PM in CAPs-exposed mice scores of (2.00±0.94 vs. 0.20±0.42, respectively, P0.001). PM exposure increased IL-6 secretion up to seven-fold in a dose-dependent manner by isolated wild-type but not TLR4-/- Kupffer cells (P0.050). In conclusion, ambient PM2.5 exposure may be a significant risk factor for NAFLD progression.