An osmotic induction method for externally marking saltwater fishes, Stigmatopora argus and Stigmatopora nigra, with calcein

摘要

Calcein marking via osmotic induction and overnight immersion was compared in saltwater fishes. Immersion in a salt solution for 10 min followed by 30 min in a 500 mg l-1 calcein solution produced the highest fluorescent mark in 40 min with no effect on survival.