Effects of culture conditions on production of antibiotically active metabolites by the marine alga Spyridia filamentosa (Ceramiaceae, Rhodophyta). I. Light

摘要

The effect of light (5-70μmol m-2 s-1) on the production of antibiotically active metabolites was assessed in male and female gametophytes and tetrasporophytes of S. filamentosa (collected from Puerto Rico) in culture. Whole-algal extracts and 10 recognizable TLC-separable zones were assayed against 5 microorganisms pathogenic to man. For all light conditions, the 10 TLC zones displayed activity against 4 of the microorganisms. The highest number of TLC zones with activity under any of the cultureconditions was 6 each for male and female S. filamentosa at the highest irradiance. Small changes in irradiance resulted both in different activities against specific microorganisms and degree of activity. The fact that every TLC zone showed differing activities at different light conditions or when extracted from different life history stages strongly suggested the presence of multiple antibiotic compounds in individual TLC zones.