Identification of the Gene Encoding a NAD(P)H-Flavin Oxidoreductase Coupling with Dibenzothiophene (DBT)-Desulfurizing Enzymes from the DBT-Nondesulfurizing Bacterium Paenibacillus polymyxa A-1

摘要

The gene encoding NAD(P)-H-flavin oxidoreductase (flavin reductase), which couples efficiently with dibenzothiophene (DBT)-desulfurizing monooxygenases of Rhodococci, was cloned from a DBT-non-desulfurizing bacterium Paenibacillus polymyxa A-1 in Escherichia coli, and designated as flv. Cell-free extracts from the recombinant exhibited a flavin reductase activity about forty times higher than that of the E. coli carrying the vector DNA only. Nucleotide sequence analysis reveals that the gene product consists of 208 amino acids and showed about 27%, 32% and 21% identity in amino acid sequence with FRase I, the major flavin reductase of Vibrio fischeri, the NADH dehydrogenase of Thermus thermophilus and several members of the nitroreductase family, respectively. The coexpression of flv with tow kinds of desulfurizing genes, dszABC and tdsABC, in E. coli enhanced the rate of DBT degradation by about 10 and 5 times as high as in the case without flv, respectively.