Composition and Cell Cytotoxicity of Essential Oil from Caryopteris incana Miq. in Korea

摘要

The essential oil was obtained from the aerial part of Caryopteris incana Miq. by steam distillation, samples were collected by headspace (HS) and solid-phase microextraction (SPME) methods, and the compositions of the essential oil were analyzed by gas chromatography-mass spectrometry (GC-MS). The fragrance of the essential oil was fougere and woody. There were sixty-nine constituents in the essential oil: 28 carbohydrates, 22 alcohols, 7 acetates, 7 ketones, 3 aldehydes, and 2 others. Major constituents were 4,6,6-trimethyl [15-(1alpha,2(3,5alpha)]-bicyclo[3.1.1]hept-3-en-2-ol (11.8%), tau-cadinol (9.4%), myrtenyl acetate (9.2%), pinocarvone (7.0%), l-hydroxy-l,7-dimethyl-4-isopropyl-2,7-cyclodecadiene (6.3%), 5-3-carene (6.2%). By SPME extraction, forty-nine constituents were identified: 22 hydrocarbons, 16 alcohols, 6 acetates, 3 ketones, and 2 ethers. Major constituents of the SPME-extracted sample were 5-3-carene (12.6%), (-)-myrtenyl acetate (11.2%), 6,6-dimethyl-2-methylene-bicycol [3.1.1]heptan-3-ol (10.9%), pinocarvone (9.3%). By HS extraction, ten constituents were identified: 5 hydrocarbons, 2 amines, 1 alcohol, and 2 others. Major constituents of the HS-extracted sample were (Z)-2-fluoro-2-butene (34.9%), 8-3-carene (6.9%), 6-(4-chlorophenul)tet-rahydro-2-methyl-2H-l,2-oxazine (5.9%). The IC_(50) value (0.011 mu g/mg) m MTT assay using HaCaT keratinocyte cell line was lower than those of commercially-selling rosemary and tea tree, suggesting more toxicological studies are needed forcommercial use of the essential oil of Caryopteris incana Miq.