Rhizobium phenanthrenilyticum sp. nov., a novel phenanthrene-degrading bacterium isolated from a petroleum residue treatment system

摘要

Strain F11~T, a phenanthrene-degrading bacterium, was isolated from a petroleum residue treatment system, and classified under the genus Rhizobium based on the similarity analysis of its 16S rRNA and recA gene sequences. Strain F11~T falls into the same phylogenetic clade with Rhizobium oryzae Alt 505~T (96.8% 16S rRNA gene sequence similarity) and Rhizobium pseudo-ryzae J34A-127~T (96.2%). Major cellular fatty acids of strain F11~T are C_(16:0) (6.24%) and summed feature 8 (C_(18:1ω7c) and/or C_(18:1ω6c), 76.59%), which are also the major fatty acids of R. oryzae Alt 505~T and R. pseudoryzae J34A-127~T. The DNA G+C content of strain F11~T was 59.3±0.4 mol%. Based on the phylogenetic analysis as well as biochemical and physiological characteristics, strain F11~T could be separated from all recognized Rhizobium species. Strain F11~T (=DSM 21882~T=CCTCC AB 209029~T) was considered to be representative of a novel species of Rhizobium, for which the name Rhizobium phenanthrenilyticum sp. nov. is proposed.