GC-MS Analysis of Headspace and Liquid Extracts for Metabolomic Differentiation of Citrus Huanglongbing and Zinc Deficiency in Leaves of 'Valencia' Sweet Orange from Commercial Groves

摘要

Introduction - Citrus Huanglongbing (HLB) is considered the most destructive citrus disease worldwide. Symptoms-based detection of HLB is difficult due to similarities with zinc deficiency.Objective - To find metabolic differences between leaves from HLB-infected, zinc-deficient, and healthy 'Valencia' orange trees by using GC-MS based metabolomics.Methodology - Analysis based on GC-MS methods for untargeted metabolite analysis of citrus leaves was developed and optimized. Sample extracts from healthy, zinc deficient, or HLB-infected sweet orange leaves were submitted to headspace solid phase micro-extraction (SPME) and derivatization treatments prior to GC-MS analysis.Results - Principal components analysis achieved correct classification of all the derivatized liquid extracts. Analysis of variance revealed 6 possible biomarkers for HLB, of which 5 were identified as proline, beta-elemene, (-)trans- caryophyllene, and alpha-humulene. Significant (P < 0.05) differences in oxo-butanedioic acid, arabitol, and neo-inositol were exclusively detected in samples from plants with zinc deficiency. Levels of isocaryophyllen, alpha-selinene, beta-selinene, and fructose were significantly (P < 0.05) different in healthy leaves only.Conclusion - Results suggest the potential of using identified HLB biomarkers for rapid differentiation of HLB from zinc deficiency. Copyright (C) 2010 John Wiley & Sons, Ltd.