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Diversity Assessment of Turkish Maize Landraces Based on Fluorescent Labelled SSR Markers

机译:基于荧光标记SSR标记的土耳其玉米地方品种多样性评估

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摘要

Landraces of maize represent a valuable genetic resource for breeding and genetic studies. Since 1970, landraces have been collected from all over Turkey, but the genetic diversity represented in this collection is still largely unknown. In this study, a sample of 98 landraces sampled from 45 provinces of Turkey was assessed genotypically at 28 simple sequence repeat (SSR) loci and phenotypically for 19 morphological traits. The landraces varied significantly for all the latter traits. A total of 172 SSR alleles were detected, giving a mean of 6.21 alleles per locus. The genetic distance between pairs of landraces ranged from 0.18 to 0.63, with a mean of 0.35. Positive and negative correlation exists among different morphological and agronomic traits. Positive association among different traits showed that improvement of one character may simultaneously improve the other desired trait. Based on UPGMA dendrogram and Neighbor-Net (NNET) analyses from both morphological traits and SSR data, respectively, it is obvious that maize landraces from the same geographical region were often placed in different clusters, indicating that grouping based on genetic parameters was not closely related to the geographic origin. The wide diversity present in Turkish maize landraces could be used as genetic resource in designing maize breeding program for developing new cultivars adapted to different geographic and climatic conditions, and may also contribute to worldwide breeding programs.
机译:玉米的地方品种是育种和遗传研究的宝贵遗传资源。自1970年以来,已经从土耳其各地收集了地方品种,但是该品种所代表的遗传多样性仍然未知。在这项研究中,从土耳其的45个省采样的98个地方品种样本在28个简单序列重复(SSR)位点进行了基因型评估,并在表型上评估了19个形态特征。对于所有后一种特性,地方品种差异很大。总共检测到172个SSR等位基因,平均每个位点有6.21个等位基因。成对的地方品种之间的遗传距离为0.18至0.63,平均为0.35。不同形态和农艺性状之间存在正相关和负相关。不同性状之间的正相关表明,一个性格的提高可能同时改善另一个所需性状。基于UPGMA树状图和NNET,分别从形态特征和SSR数据进行分析,很明显,来自同一地理区域的玉米地方品种通常被放置在不同的集群中,这表明基于遗传参数的分组并不紧密与地理起源有关。土耳其玉米地方品种中存在的广泛多样性可以用作设计玉米育种计划的遗传资源,以开发适应不同地理和气候条件的新品种,也可以为全球育种计划做出贡献。

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