The inheritance of buckwheat (Fagopyrum esculentum Moench) seed storage proteins was investigated by control crosses between accessions containing different SDS-PAGE protein patterns in the range of 38-48 kDa. Analysis of segregation of individual F and F seeds obtained by 17 crosses showed segregation of eight electrophoretic bands, from which 12-banding variants were inferred. Two variants contained one band, nine contained two bands and one variant contained three bands and behaved as a single co-dominant unit. The segregation of banding variants fits the expected ratios and thus supports the hypothesis of single Mendelian gene inheritance and that alleles are co-dominant. The results therefore suggest that the genes controlling globulin subunits are tightly linked and inherited as a single locus. The locus was designated Glob-1 and the twelve segregated protein variants as alleles a-l. This is the first buckwheat globulin locus identified with multiple alleles, phenotypically expressed as groups of protein bands and thus applicable as a functional marker in buckwheat genomic studies.
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