EFFECTS OF EXOGENOUS AND ENDOGENOUS HYDROGEN SULFIDE ON PLASMA RENIN AND ERYTHROPOIETIN IN CISPLATIN-INDUCED NEPHROTOXICITY IN RAT

摘要

Background & Aims : Hydrogen sulfide due to cytoprotective effects can be used in prevention and treatment of many disorders. The effects of hydrogen sulfide onhormonal system in rat kidneys with cisplatin (CP) nephrotoxicity are unknown. The purpose of the experiments was to survey the effects of hydrogen sulfide on rat kidneys hormonal system with cisplatin nephrotoxicity. Materials & Methods : In this experimental study, sixty male Sprague-Dawley rats were divided into six groups: control group (C), nephrotoxic group received single dose of cisplatin (CP, 6mg/kg, i.p.), nephrotoxic groups that received single dose NaHS (100μmol/kg, i.p.), propargyl glycine (PAG=CSE inhibitor, 50 mg/kg, i.p.), PAG+CP and PAG+NaHS+CP. Animals were sacrificed 6 days after CP (or vehicle) treatment, and blood was collected for laboratory analysis. Results: The results showed that plasma urea and creatinine in cisplatin nephrotoxic rats were significantly higher than the control group. Plasma erythropoietin in cisplatin nephrotoxicity group increase d when compared to the control group. There was a slight increase in plasma renin concentration in the PAG group when compared to other groups. When the hydrogen sulfide was administered to the experimental rats, these changes were reversed. Conclusion: Hydrogen sulfide prevented erythropoietin, urea, and creatinine changes in cisplatin nephrotoxic rats probably by its cytoprotective effects such as antioxidant properties.