Abnormal expressions of signal molecules PTEN, FAK and Shc after implantation of golden hamster Mesocricetus auratus embryos in mouse Mus musculus Uteri (In English)

摘要

Interspecific embryos always abort at certain phase in pregnancy. The majority of previous studies focused on descriptions of macrostructure and anatomic differences and molecular mechanisms of these phenomena were rarely studied. In order to elucidate the possible roles of signal molecules in failure of interfamily pregnancy, we compared the expressive differences of integrin alpha v beta 3, FAK, p-ERK, IGFⅡ, IGFBP-1, Shc, PTEN and MMP-2, -9 between interfamily and intraspecific fetuses by immunohistochemistry and gelatin zymography. Interfamily pregnancy was achieved by transferring embryos of golden hamster Mesocricetus auratus into pseudopregnant mouse Mus musculus uteri. Interfamily pregnant fetuses of day 4, 8 and 12 formed the experimental group. The intraspecific pregnant mice uteri after implantation of mice embryos to the uteri of pseudopregnant mice at corresponding time provided positive and negative controls, respectively. The immunohistochemical results showed that the expressions of integrin αvβ3, IGF-Ⅱ, IGFBP-1, PTEN, Shc and FAK of interfamily fetuses on day 8 were all different to those of normal intraspecific pregnancy day 8. While the expressive localization and intensity of p-ERK both had no evidently different between interfamily and interspecific pregnancy on day 8. Gelatin zymography indicated obvious differences of secretive modes of MMP-2 and -9 between interfamily and intraspecific pregnancy. The results suggested that these signal molecules differentially expressed in uteri participate to the formation of abnormal histological exhibitions in maternal-fetal interface of interfamily pregnancy[ Acta Zoologica Sinica 51(6): 1067–1072,2005].