Studies revealing bioremediation potential of the strain Burkholderia sp. GB-01 for abamectin contaminated soils

摘要

Burkholderia sp. GB-01 strain was used to study different factors affecting its growth for inoculum production and then evaluated for abamectin degradation in soil for optimization under various conditions. The efficiency of abamectin degradation in soil by strain GB-01 was seen to be dependent on soil pH, temperature, initial abamectin concentration, and inoculum size along with inoculation frequency. Induction studies showed that abamectin depletion was faster when degrading cells were induced by pre-exposure to abamectin. Experiments performed with varying concentrations (2–160 mg Kg−1) of abamectin-spiked soils showed that strain GB-01 could effectively degrade abamectin over the range of 2–40 mg Kg−1. The doses used were higher than the recommended dose for an agricultural application of abamectin, taking in account the over-use or spill situations. A cell density of approximately 108 viable cells g−1 dry weight of soil was found to be suitable for bioremediation over a temperature range of 30–35°C and soil pH 7.5–8.5. This is the first report on bacterial degradation of abamectin in soil by a Burkholderia species, and our results indicated that this bacterium may be useful for efficient removal of abamectin from contaminated soils.