Indole degrading of ammonia oxidizing bacteria isolated from swine wastewater treatment system

摘要

Three new strains named LPA11, LPB11 and LPC24 were isolated to investigate the patterns of indolendegradation and ammonia oxidation in swine wastewater from different parts of a swine wastewaterntreatment system by the direct spreading plate method. These three isolates were all identified asnPseudomonas putida based on 16S-rDNA gene sequences, main physiological and biochemicalnanalysis. They were capable of decomposing 1.0mM indole completely in 10, 16 and 18 daysnrespectively. According to the results of HPLC and GC/MS, the possible pathway for the degradationnwas via oxindole, isatin and anthranilic acid. The three bacteria were capable of oxidizing ammonia,nand the strains LPA11 and LPC24 were capable of effectively reducing nitrate and nitrite.