Microbial community structure and occurrence of diverse autotrophic ammonium oxidizing microorganisms in the anammox process

摘要

The enrichment of anaerobic ammonium oxidizing (anammox) bacteria using an upflow anaerobicnsludge bioreactor was successfully conducted for 400 days of continuous operation. The bacterialncommunity structure of anammox bioreactor included Proteobacteria (42%), Chloroflexi (22%),nPlanctomycetes (20%), Chlorobi (7%), Bacteroidetes (5%), Acidobacteria (2%), and Actinobacterian(2%). All clones of Planctomycetes were affiliated with the anammox bacteria, PlanctomycetenKSU-1 (AB057453). The presence and diversity of ammonia oxidizing bacteria (AOB) and archaean(AOA) were identified by terminal restriction fragment length polymorphism (T-RFLP) based on thenamoA gene sequences. The AOB in anammox bioreactor were affiliated with the Nitrosomonasneuropaea cluster. The T-RFLP result of AOA showed the diverse microbial community structurenof AOA with three terminal restriction fragments (T-RFs).