Stimulating the anaerobic biodegradation of explosives by the addition of hydrogen or electron donors that produce hydrogen

摘要

The anaerobic biodegradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), and 2,4,6-trinitrotoluene (TNT) by a methanogenic mixed culture was investigated. Microcosms containing a basal medium and the mixed culture were amended with ethanol, propylenc glycol (PG), butyrate or hydrogen gas as the electron donor and a mixture of TNT (50 μM), RDX (25 μM), and HMX (8 μM). After 29 days TNT and RDX were completely transformed to unidentified endproducts in the bottles amended with ethanol, hydrogen, or PG, while 53%, 40%, and 22% of the HMX was transformed, respectively. There was no loss of RDX or HMX in the electron donor unamended control bottles. The ethanol and PG were transformed to near stoichiomelric amounts of acetate and propionate, suggesting the immediate electron donor supporting the transformation of the explosives was the H_2 evolved during the metabolism of the parent substrate. Our findings suggest that the addition of H_2 or electron donors that produce H_2 may be a useful strategy for enhancing the anaerobic biodegradation of explosives in contaminated groundwater and soils.