Confocal microscope employs digital light projector

摘要

In fluorescence microscopy, specimens are illuminated by a specific wavelength of light that is absorbed by the fluorophores in the sample and emitted at a longer wavelength. In conventional microscopes, this emitted fluorescence can be from a relatively wide focused background area of the sample, with the result that the fluorescence that is required to be measured at a single point is obscured by fluorescence from out of focus points in the sample. To overcome this interference, confocal imaging techniques increase the contrast of fluorescence images by employing point illumination and a spatial pinhole to eliminate any out of focus fluorescence.