Aequorea victoria bioluminescence moves into an exciting new era

摘要

Bioluminescence has revolutionized research into manycellular an molecular-biological processes, ranging from intracellu- lar signalling to gene transcription. This article focuses on the chemistry and biotechnological exploitation of the two proteins invollved in bioluminescence of the jellyfish Aequorea victoria-aequorin and green fluorescent protein. Engineered recombi- nant aequorin has led to a novel technological approach to monitoring calcium signals in organelles and subcellular domains.