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Assessment of genetic diversity, population structure, and phylogenetic relationships among the Northeast Indian and South Indian commercially released tea cultivars using TE-AFLP markers

机译:使用TE-AFLP标记评估东北印第安人和南印第安人商业发布的茶品种之间的遗传多样性,种群结构和系统发育关系

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Commercially cultivated tea clones from Northeast and Southern India were analyzed using TE-AFLP markers. Analysis of molecular variance (AMOVA) and structure analysis showed high genetic variance (74%) within five distinct populations. This study also confirmed that Assam and UPASI (Nilgiri) tea clones have originated from two different tea populations introduced from imported China tea seeds by British. We evaluated the genetic variation and population structure in 30 tea clones of Tea Research Association (TRA), Tocklai Experimental station (Tockali ES), Jorhat, from North Eastern India and 30 clones of United Planters Association of South India (UPASI), Valparai, from South-India, released for commercial plantations and four C. japonica accessions (Total 64) used as outliers using Three Endonuclease-AFLP markers. Six primer combinations were selected from the best 10 primer combinations that were used on a set of 24 tea accessions as a standardization procedure. Dendrograms were constructed by using Jaccard's similarity coefficient and Unweighted Pair Group Method of Arithmetic Averages (UPGMA) clustering method based on high coefficient correlation ratios obtained. High level of genetic diversity and clustering of accessions according to morphological proximity was established with minor aberrations. Analysis of molecular variance (AMOVA) showed highest genetic variance (79%) within five populations whereas the lowest variance (21%) was found among populations. These values change to the highest 87% and the lowest 13% when Camellia japonica accessions were removed from the analysis. Population structure analyses were performed to establish the genetic diversity and exact type each clones vis-a-vis to their molecular and morphological characteristics at present. Attempts were made to find out the population structure of Northeast and Southern India tea germplasm with their relatedness to existing morphological type. These analyses confirmed that all tea clones were genetically diverse and grouped based on their morphological characteristics and geographic regions.
机译:使用TE-AFLP标记分析了印度东北和南部商业种植的茶克隆。分子变异分析(AMOVA)和结构分析显示,五个不同种群具有较高的遗传变异(74%)。这项研究还证实,阿萨姆邦和UPASI(Nilgiri)茶克隆起源于英国从中国进口的茶种中引入的两种茶种群。我们评估了印度东北部Jorhat茶研究协会(TRA),Tocklai实验站(Tockali ES)的30个茶无性系和南印度联合种植者协会(UPASI)的30个茶无性系的遗传变异和种群结构。来自南印度的植物,已发布用于商业种植园,并使用三个核酸内切酶-AFLP标记用作离群值的四个粳稻种(总计64)。从最佳的10种引物组合中选择6种引物组合,将其作为一组标准化程序用于24个茶种。基于获得的高系数相关比,使用Jaccard的相似系数和算术平均值的非加权对群方法(UPGMA)聚类方法构造树状图。建立了高水平的遗传多样性和根据形态接近性的种质聚类,且畸变较小。分子变异分析(AMOVA)显示五个种群中最高的遗传变异(79%),而种群中最低的变异(21%)。从分析中删除山茶花后,这些值最高变为87%,最低变为13%。进行种群结构分析以建立目前每个克隆相对于其分子和形态特征的遗传多样性和确切类型。试图找出印度东北和印度南部茶种质的种群结构及其与现有形态类型的关系。这些分析证实,所有茶克隆都具有遗传多样性,并根据其形态特征和地理区域进行了分组。

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