SYNTHESIS, SPECTROSCOPIC STUDIES, ANTIBACTERIAL ACTIVITY OF CHALCONES AND COLORIMETRIC EVALUATION OF THE TIME-KILLING ASSAY FOR NEWLY SYNTHESIZED CHALCONES USING RESAZURIN

摘要

An attempt was made here to synthesize and screen the chalcones against certain Gram positive and Gram negative bacteria by adopting the modified colorimetric technique for Time-Killing Assay. A series of chalcones were synthesized from 1-(2-bromophenyl) ethanone and different substituted aromatic aldehydes with good yield and resolute on the basis of spectral data (FT/1R, ~1H NMR and ~(13)C NMR). The synthesized chalcones were screened for antibacterial activity against certain Gram positive and Gram negative bacteria. The pharmacokinetic profiles of chalcones were established by Time-Kill Assay, performed by colorimetry using resazurin dye as an indicator which colors only the viable cells. The ethoxy (1-4) and parahydroxy (1-5) derivatives were found to be more potent against Gram positive bacteria. The colorimetric Time-Kill Assay was found to be more convenient and less time consuming than traditional cell viable count.