Comparative studies on ω-hydroxylation of 5β-cholestane-3α, 7α ,12α-triol in the mitochondrial and microsomal fraction of the liver from several vertebrates

摘要

The activity and the stereospecificity of ω-hydroxylation, a hydroxylation at one of the two terminal methyl groups of 5β-cholestane-3α, 7α, 12α-triol, which is thought to be the first step in side-chain degradation resulting in the formation of cholic acid, was elucidated in mitochondria and microsomes of the liver from several evolutionarily primitive vertebrates, fish frogs turtles, and chickens in addition to such mammals as rats, hamsters, and rabbits. The detection of ω-hydroxylation products (25R)- and (25S)-5β-cholestane-3α, 7α, 12α, 26-tetrols as well as the separation of their two isomers was facilitated using high-performanee liquid chromatography after conversion to 9-anthroyl derivatives.