Molecular imprinting technique in putrescine visualized detection

摘要

Highlights•The specific adsorption of putrescine on MIP is visualized by applying chromogenic agent.•The color stain on MIP is subjected to peak-differenating analysis and quantified by visible spectrum.•3D interactive surface plot is generated by color stains on MIP, providing an intuitive representation of the vapor spatial shape.AbstractThis work is an exploration into visualizing measurement of putrescine specific adsorption by photographing colorized molecule imprinting chromogenic hydrogel. Membranes are prepared with imitate templates 1,4-butylene glycol, adipic acid or succinic acid, respectively and ninhydrin is used as chromogenic agent for target molecule putrescine. The adsorbing concentration on MIP is reflected in the form of visible violet-colored stain differed in shape and shade, which are recorded by hyper spectral camera and proceeded into intuitive 3D isohypse surfaces plot. By quantifying the height of surface peaks, imprinting efficiency is estimated in accordance with polyvinyl alcohol concentration and crosslinking degree. The imprinting efficiency of three imitate molecule templates is also discussed.