Hemoglobin-Mn_3(PO_4)_2 hybrid nanoflower with opulent electroactive centers for high-performance hydrogen peroxide electrochemical biosensor

摘要

In our study, flower-like hemoglobin (Hb)-Mn_3(PO_4)_2 hybrid nanoflowers (HMHNFs) were synthesized to generate a hydrogen peroxide (H_2O_2) electrochemical biosensor. Hybrid structure consisted of Hb and Mn_3(PO_4)_2 was designed for the first time to enhance the electrochemical signal of biosensor. This new electrochemical sensing material, can efficiently catalyze the reduction of H_2O_2, amplify electrochemical signal and increase sensitivity. This new designed biosensor exhibited an outstanding electrochemical performance in the selective and sensitive measurement of H_2O_2 with an extremely low detection limit of 7 nM and the linear range was from 20 nM to 3.6 μM. The excellent H202-sensing performance can be attributed to the unfolding of Hb as well as the high specific surface of HMHNFs that produces opulent exposed electroactive centers for H_2O_2 reduction. Moreover, HMHNFs-based biosensor performed well in the measurement of H_2O_2 in both rainwater and human serum samples. These results indicated HMHNFs-based biosensor was a potential candidate in clinical analysis and environmental monitoring.