In vivo bio-imaging studies of highly selective, sensitive rhodamine based fluorescent chemosensor for the detection of Cu~(2+)/Fe~(3+) ions

摘要

A Rhodamine based quinoline conjugated fluorescence chemosensor (RhQA) was delineated for the detection of two paramagnetic metal ions (Fe3+/Cu2+) through various analytical techniques. The addition of Fe3+and Cu2+ions induced a great enhancement in the absorption of RhQA; moreover, Fe3+also induced a strong fluorescence enhancement. Hence, this is an admirable example for the selective detection of the biologically/environmentally significant transition metal ions as dual chemosensor. The obtained results reveal that the chemosensor (RhQA) has remarkable selectivity towards Fe3+/Cu2+ions even in the presence of higher concentration of various interference metal ions such as Pb2+, Cd2+, Hg2+, Zn2+, Ni2+, Co2+, Mn2+, Fe2+, Cr3+, Ca2+, Ba2+, Mg2+, Na+, K+, and Ag+. Its excellency in sensitivity was confirmed by the obtained linear relationship with Fe2+/Cu3+ions, and the calculated detection limit values are, 1.8 × 10−8and 3.3 × 10−8M for Cu2+and Fe3+ions respectively. More interestingly, this chemosensor, RhQA was successfully employed to trace out the intra cellular Fe3+ions in Zebrafish embryos.