AUTOMATED LIBRARY CONSTRUCTION

摘要

Spriworks Fragment Library System III automates the preparation of libraries for the Life Technologies SOLiD Sequencer. With the introduction of this third system in a series, researchers can use the SPRIworks system to automate library preparation for the three major next generation sequencing platforms. Combining Solid Phase Reversible Immobilization (SPRI) chemistry and Beckman Coulter automation, SPRIworks System III facilitates the construction of consistent, quality libraries. Automating the library construction process saves researchers valuable time. Between one and 10 samples can be completed within three and a half hours, and the process provides several size-selection and nick-translation options. SPRIworks Fragment Library System III is a validated plug-and-play system: users simply insert the method card, samples, and adapters; select the run options; and press start.