Occurrence and fate of antibiotic residues and antibiotic resistance genes in a reservoir with ecological purification facilities for drinking water sources

摘要

Antibiotics and antibiotic resistance genes (ARGs) are emerging contaminants in surface waters, especially drinking water sources, where they can pose a risk to human health and aquatic ecology. The ecological purification facilities constructed in the drinking water reservoirs are intended to safeguard water quality. However, their ability to remove antibiotics and ARGs from the water and the presence of ARGs in such river-reservoir systems have not been comprehensively characterized yet. The occurrence, distribution and spatiotemporal variations in antibiotics, ARGs, and mobile genetic elements (MGEs) were investigated in a representative river-reservoir system in Shanghai, China. A total of 283 ARGs and 12 MGEs were detected in the water using high- throughput quantitative PCR analysis. Antibiotic residues and the absolute abundance of total ARGs and MGEs in reservoir inflow were significantly reduced when water from the river passed through the ecological purification processes in the reservoir. Antibiotics in this river-reservoir system posed only limited risks to the aquatic ecosystem and human health. No significant correlation was observed between the distribution pattern of ARGs and spatiotemporal factors. The dominant ARGs were strongly and significantly correlated with integrons. Through redundancy analysis and variation partitioning analysis, we determined that MGEs were the major driver shifting the distribution of ARGs, and the effects of environmental factors and antibiotic residues were reflected in the joint effects with MGEs. The small ecological reservoir was verified as an effective engineering to mitigate ecological risk in the drinking water source.