Gene controlled by promoter-P_(TH4) depending on whiG of Streptomyces coelicolor

摘要

The downstream gene controlled by promoter-P_(TH4) which is related to Streptomyces differentiation was cloned, and its sequence was determined by the dideoxy chain termination method. The re-suits indicated that the 1 597 bp of DNA fragment conferred a complete open reading frame (ORF). In searches of databases, the deduced product of the ORF was not homologous with any known proteins; it may be a new protein. The function of the gene was studied using the strategy of gene disruption; the actinorhodin could not be produced when this gene was disrupted. Therefore, this gene may be related to actinorhodin biosynthesis in Streptomyces coelicolor, and the result also shows that this gene may play a role in multiple level regulation of differentiation genes in Streptomyces.