Screening of proteins that interact with human thromboposietin receptor c-Mpl using yeast two-hybrid system

摘要

Thrombopoietin (TPO) is the major cytokine involved in platelet production and exerts its effects via the receptor c-Mpl. The yeast two-hybrid system has been used to screen th pro- teins interacting with c-Mpl. First, the cDNA fragment of c-Mpl intracellular domain was cloned into two-hybrid vector pAS2, and the resulting plasmid is designated as pASMM. Then a human pla- centa cDNA library was screened using the pASMM as a target plasmid.