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Characteristics of the microbial community in rhizosphere of Camptotheca acuminata cultured with exotic invasive plant Eupatorium adenophorum

机译:外来入侵植物紫茎泽兰培养的喜树根际微生物群落特征

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The traditional culture-dependent plate counting and culture-independent small-subunit-ribosomal RNA gene-targeted molecular techniques, Single-Strand Conformation Polymorphism (SSCP) and terminal Restriction Fragment Length Polymorphism (tRFLP) combined with 16S rDNA clone library were adopted to investigate the impacts of secretion from Camptotheca acuminata (abbreviated to Ca) roots on the quantities and structure of eukaryotic microbes and bacteria in the rhizosphere, and the possibility that Ca controls exotic invasive plant Eupatorium adenophorum (Ea). The counting results indicated that the number of bacteria increased in turn in rhizospheres of Ea, Ca-Ea mixed culture and Ca, while that of eukaryotic microbes decreased. PCR-SSCP profiles showed eukaryotic microbial bands (corresponding to biodiversity) in rhizosphere of Ea were more complex than those of Ca and CE. Meristolohmannia sp., Termitomyces sp. and Rhodophyllus sp. were the dominant populations in the rhizosphere of Ca. Bacterial terminal restriction fragments (TRFs) profiles showed no difference among three kinds of rhizospheres, and the sequences of the 16S rDNA clone library from Ca rhizospheres were distributed in 10 known phyla, in which phylum Proteobacteria were the absolute dominant group and accounted for 24.71% of the cloned sequences (5-Proteobacteria accounted for up to 17.65%), and phyla Acidobacteria and Bacteroidetes accounted for 16.47% and 10.59% of the cloned sequences, respectively. In addition, high performance liquid chromatography detected a trace amount of camp tot hecin and hydroxycamptothecin in the rhizospheric soil of Ca and CE, but examined neither camptothecin nor hydroxycamptothecin in rhizospheric soil of Ea. Therefore, invasion and diffusion of Ea evidently depended on distinguishing the eukaryotic community structure, but not on that of the bacterial pattern. Ca was able to alter the eukaryotic community structure of invasive Ea by secreting camptothecin and hydroxycamptothecin into rhizospheres, and may benefit the control of overspread of Ea. This study provided theoretical evidence for rhizospheric microbial aspects on substituting Ca for Ea.
机译:采用传统的依赖培养的板计数和不依赖培养的小亚基核糖体RNA基因靶向分子技术,单链构象多态性(SSCP)和末端限制性片段长度多态性(tRFLP)结合16S rDNA克隆文库进行研究。喜树根(缩写为Ca)的分泌物对根际中真核微生物和细菌的数量和结构的影响,以及Ca控制外来入侵植物紫茎泽兰(Euatorium Adenophorum)的可能性。计数结果表明,Ea,Ca-Ea混合培养物和Ca的根际细菌数依次增加,而真核微生物数减少。 PCR-SSCP图谱显示,Ea根际的真核微生物带(与生物多样性相对应)比Ca和CE更复杂。 Meristolohmannia sp。,Termitomyces sp。和Rhodophyllus sp。是Ca根际的优势种群。三种根际之间的细菌末端限制性片段(TRFs)图谱没有差异,来自Ca根际的16S rDNA克隆文库的序列分布在10个已知门中,其中Proteobacteria是绝对优势群,占24.71%克隆序列中的5个(最多占到7.65%),酸性细菌和拟杆菌属分别占克隆序列的16.47%和10.59%。另外,高效液相色谱法在Ca和CE的根际土壤中检测到痕量的喜树碱和羟基喜树碱,但在Ea的根际土壤中均未检测到喜树碱和羟基喜树碱。因此,Ea的入侵和扩散显然取决于区分真核生物群落结构,而不取决于细菌的模式。 Ca能够通过将喜树碱和羟基喜树碱分泌到根际中来改变侵入性Ea的真核生物群落结构,并可能有利于控制Ea的过度扩散。该研究为根际微生物方面用Ca替代Ea提供了理论依据。

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