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Simple, Automatic, Continuous‐Recording Respirometer

机译:简单,自动,连续记录的呼吸仪

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A new type of respirometer is described. The method involves a ``diver'' which is composed of a rigid respiratory chamber containing a CO2 absorbent and an O2‐containing collapsible sack feeding O2 into the chamber by way of a capillary tube. As O2 is used by the organism in the respiratory chamber, replacement O2 arrives from the collapsible sack thereby decreasing the buoyancy of the system. The change in buoyancy is recorded on a kymograph drum by an ink‐recording spring scale. The method has been put in practice using 50 ml Soxhlet distilling flasks with rubber stoppers penetrated by 27‐gauge hypodermic needles as the respiratory chamber and sections of Saran tubing (Visking Corporation of Chicago) with an O2 capacity of 40–50 ml attached to the stopper as the collapsible sacks. Recording spring scales with variable ranges from 2 to 20 g which have served satisfactorily for measuring natural variations in rates of O2 consumption for certain small animals are described.
机译:描述了一种新型的呼吸计。该方法涉及一个``潜水员'',该潜水员由一个装有CO2吸收剂的刚性呼吸腔和一个通过毛细管将O2注入腔中的含O2的可折叠袋组成。由于氧气被呼吸腔内的生物体所利用,替换氧气从可折叠袋中到达,从而降低了系统的浮力。浮力的变化通过墨水记录弹簧刻度记录在运动鼓上。该方法已在实践中使用了50毫升的索氏抽提瓶,其中的橡胶塞被27号皮下注射针刺入作为呼吸腔,并且Saran管(芝加哥维斯金公司)的部分与O-2容量为40–50 ml相连。塞子是可折叠的麻袋。描述了记录范围从2到20 g的弹簧秤,该弹簧秤已令人满意地用于测量某些小型动物的O2消耗速率的自然变化。

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