Identification of genes regulated by an interaction between v3 integrin and vitronectin in murine decidua

摘要

The delicate balance between embryo invasion and suppression of maternal immune rejection requires a fullynfunctional decidua in species with haemochorial placenta. Our understanding of the decidual function is very limited duento the molecular and cellular complexity involved in decidualisation. The cell adhesion molecule αvβ3 integrin and itsnligand vitronectin are upregulated in the mouse decidua during mid-pregnancy. The implications of interactions betweennαvβ3 and vitronectin in regulating decidual function are not known. In the present study, interactions between αvβ3 andnvitronectin in the decidual cells of the mouse were blocked in vitro and effects on cell fate were evaluated by studyingnthe differentially regulated genes by cDNA array and real-time polymerase chain reaction (PCR). The results indicate thatnexpression of various genes involved in apoptotic and cell cycle pathways, as well as cytokine receptors, was deranged.nSignalling through αvβ3 seems to be important to maintain a balance between cell proliferation and apoptosis, along withnthe modulation of inflammatory responses of decidual cells.