Study on consolidated bioprocessing of pre-treated Nannochloropsis gaditana biomass into ethanol under optimal strategy

摘要

Nannochloropsis gaditana is a microalga, constituting cellulose 33 +/- 1.3% and hemicellulose 18.2 +/- 0.8%; used for the biofuel production. The cellulose in microalgal biomass would contribute to protecting the environment and constitute an attractive renewable raw material for bioethanol production. Ethanol production from N. gaditana microalgal biomass was investigated through Consolidated Bioprocessing (CBP) using Hangateiclostridium thermocellum KSMK1203 bacterial isolate. Screening and optimization of biomass pretreatment with different acids in different dosages, various hydrolysis time at 100 degrees C were performed and statistically validated using one-way ANOVA for efficient hemicellulose removal from NaClO2-treated N. gaditana biomass. Medium components and process parameters were statistically optimized by Response Surface Methodology. The maximum cellulase of 13.96 +/- 1.354 IU/mL and ethanol of 12.90 +/- 0.987 g/L were obtained in pretreated N.gaditana biomass. This study demonstrates that N. gaditana is a substitution for vegetal cellulose and these optimized conditions could be successfully used for commercial ethanol production using H. thermocellum KSMK1203. (c) 2021 Elsevier Ltd. All rights reserved.