Expression and Purification of Uniformly 15N-Labeled Amyloid β Peptide 1-40 in Escherichia coli

摘要

For biophysical studies using heteronuclear NMR analysis of amyloid beta peptide, construction of an efficient and high yield expression system of uniformly isotopic labeled amyloid beta peptide is desirable. Here we succeeded in obtaining 15N-labeled amyloid beta 1-40 expressed by attachment to hen egg white lysozyme as a fusion protein.