Effect of P15~(ink4b) expression on the cell cycle and G_1 phase related regulatory genes in human melanoma cells

摘要

Uing the transfection technique, P15~(ink4b) was introduced into P15~(ink4b) gene deleted human melanoma A375 cells, and a cell model MLIK6 overexpressing P15~(ink4b) was constructed. Comparing with the control cells MLC2, MLIK6 cells in G_1 phase increased by 11/100, but those in S phase decreased by 15/100 by FCM. By the method of thymidine (TDR) and N_2O arresting, the proportions of synchronized M phase cells of MLIK6 and MLC2 were measured and found to be 89.1/100 and 76.8/100, respectively, and the cells in G_1 phase were 74.3/100 for MLIK6 and 76.