Isolation and characterization of rice lesion mimic mutants from a T-DNA tagged population

摘要

A rice (Oryza sativa L. ssp japonica cv. Nipponbare) T-DNA tagged population consisting of about 7000 individual lines was generated and screened for rice lesion mimic mutants in the T_1 generation. Ten lines were found to develop spontaneous lesionsin the absence of pathogen infection and displayed distinct lesion phenotypes These mutants were tentatively designated as l1 —lm10 (for lesion mimic), respectively. Lesion formation of lm mutants was developmentally regulated, and all the mutants showed stunted growth and reduced fertility. Genetic analysis demonstrated that all the mutations were recessive, and five partially fertile mutants (lm4-—lm8) were derived from different loci. Mimic lesions occurring on the leaves of lm mutants resulted from cell death as revealed by trypan blue staining. Six of them. (lm3 — lm8) exhibited enhanced resistance to five bacterial blight isolates, indicating their wide-spectrum resistance to this pathogen, These results imply that some lesion mimic mutationsof rice might be involved in disease resistance signaling pathways, and that isolation of these mutated genes may be useful for elucidating molecular mechanisms of plant disease resistance Among the mutants, only one mutant, lm6, was preliminarily shownto cosegregate with the inserted T-DNA in its T_1 generation, making it feasible to isolate the gene responsible for the phenotype of this mutant.