Ex vivo cell labeling with ~64Cu-pyruvaldehyde-bis (N~4- methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography

摘要

We have used copper-64-pyruvaldehyde-bis(N~4-methylthiosemi- carbazone) (~64Cu-PTSM) to radiolabel cells ex vivo for in vivo positron-emission tomography (PET) imaging studies of cell traf- ficking in mice and for eventual application in patients. 2-[~18F]- Fluoro-2-deoxy-D-glucose (FDG) cell labeling also was evaluated for comparison. ~64Cu-PTSM uptake by C6 rat glioma (C6) cells in- creased for 180 min and then stabilized. The labeling efficiency was directly proportional to ~64Cu-PTSM concentration and influenced negatively by serum. Label uptake per cell was greater with ~64Cu-PTSM than with FDG. However, both ~64Cu-PTSM- and FDG- labeled cells showed efflux of cell activity into supernatant.