Lack of chromosome territoriality in yeast: Promiscuous rejoining of broken chromosome ends

摘要

Various studies suggest that eukaryotic chromosomes may occupy distinct territories within the nu- cleus and that chromosomes are tethered to a nuclear matrix. These constraints might limit interchromosomal interactions. We have used a molecular genetic test to investigate whether the chromosomes of Saccharomyces cerevisiae exhibit such territoriality. A chromosomal double-strand break (DSB) can be efficiently repaired by recombination between flanking homologous repeated sequences.