A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae

摘要

Analysis of the function of a particular gene product typically involves determining the expression profile of the gene, the subcellular location of the protein, and the phenotype of a null strain lacking the protein. Conditional alleles of the gene are often created as an additional tool. We have developed a multifunctional, transposon-based system that simultaneously generates constructs for all the above analyses and is suitable for mutagenesis of any given Saccha- romyces cerevisiae gene. Depending on the transposon used, the yeast gene is fused to a coding region for β-galactosidase or green fluorescent protein.